Boğaziçi CT3
Hizmetler
Device Name / Brand / Model
• Name: MALDI-TOF/TOF Mass Spectrometer (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight)
• Brand: Shimadzu
• Model: AXIMA PERFORMANCE
Overview
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) is an advanced mass spectrometry technique that ionizes large biomolecules—such as proteins, peptides, DNA, and polymers—and separates them based on their mass-to-charge ratios (m/z). Within targeted therapy technologies, it provides a critical analytical platform for characterization of biologics, molecular identification, and biomarker discovery. At our center, the system is actively used in multidisciplinary studies including the development of targeted drug delivery systems, biomarker validation, and molecular characterization workflows.
Purpose / Output
• m/z-based identification and molecular mass confirmation of large biomolecules and polymers
• Protein/peptide profiling and comparative proteomics analyses
• Molecular identification workflows supporting biomarker discovery and validation
• Structural elucidation via high-energy tandem MS (MS/MS) fragmentation
Application Areas
• Proteomics research: high-sensitivity identification of complex protein mixtures and comparative proteomics
• Biomolecular analyses: peptide sequencing, protein modifications, molecular mass confirmation; microbiological analyses
• Pharmaceutical research: analysis of biotech drug candidates and quality control of purified products
• Clinical research: biomarker identification, pathogen diagnosis, and biological sample analysis for personalized medicine approaches
Highlights
• Broad mass range and flexible performance via linear and reflector modes
• High sensitivity (amol level) and <5 ppm mass accuracy in reflector mode
• Structural analysis capability via high-energy tandem MS (MS/MS) using helium as collision gas
Technical Specifications
| Feature | Value / Description |
|---|---|
| Laser source | N₂ laser with variable repetition rate up to 50 Hz; near-axis laser beam for high efficiency |
| Mass range (Linear mode) | 1–500 kDa |
| Mass range (Reflector mode) | 1–80 kDa |
| Resolution (FWHM, Linear mode) | 5000 (ACTH 18–39) |
| Resolution (FWHM, Reflector mode) | 20000 (ACTH 7–38) |
| Sensitivity | 250 amol in reflector mode (Glu-fibrinopeptide) |
| Mass accuracy | <5 ppm (in reflector mode) |
| Precursor ion resolution | >400 FWHM |
| MS/MS analysis | High-energy tandem MS (MS/MS) using helium collision gas |
Contact / Service Request
For service requests: you may email [email protected]. (Please specify the sample type, purpose, and preferred date range.)