Boğaziçi CT3
Hizmetler
Device Name / Brand / Model
• Name: Laser Scanning Confocal Microscope
• Brand: Leica
• Model: STELLARIS 5
Overview
A laser scanning confocal microscope is one of the most advanced forms of fluorescence imaging technology. It uses laser beams to generate high-resolution, high-contrast images. By employing a pinhole (aperture) placed at the image plane, out-of-focus light is rejected and only light originating from the focal plane is detected, producing sharp optical sections. This system is used to analyze the localization and distribution of intracellular structures in fluorescence-labeled samples. It also enables time-dependent tracking of molecular dynamics in live cells, allowing real-time observation of dynamic biological processes. In addition, Z-stack acquisition supports 3D imaging and digital reconstruction of tissues and intracellular organelles for clearer structural analysis. With a CO₂- and temperature-controlled incubator unit, the system enables long-term observation of live-cell culture dynamics, improving efficiency and reliability in live-cell imaging experiments.
Purpose / Output
• High-resolution, high-contrast confocal fluorescence imaging of labeled samples
• Optical sectioning by collecting signal selectively from the focal plane
• 3D imaging and digital reconstruction via Z-stack acquisition
• Real-time monitoring of time-dependent dynamics in live cells (live-cell imaging)
Application Areas
• Cell and molecular biology studies
• Cancer research
• Neuroscience studies
• Developmental biology
• Immunological studies
• Pharmacology and toxicology studies
• Tissue engineering and regenerative medicine
Highlights
• Broad spectral imaging with WLL (485–790 nm) plus a 405 nm diode laser for multi-fluorophore imaging
• Advanced experiment modalities: FRAP, FLIP, photoactivation, photoconversion, and photobleaching
• Long-term live-cell imaging supported by CO₂- and temperature-controlled incubation infrastructure
Technical Specifications
| Feature | Value / Description |
|---|---|
| Laser sources | WLL Laser (485–790 nm) and Diode Laser (405 nm) |
| Multi-fluorophore imaging | Simultaneous imaging of multiple fluorescent dyes in the same sample |
| Objectives / magnification | 5X, 10X, 20X, 40X, 63X (5 objectives) |
| Experiment modalities | FRAP, FLIP, photoactivation, photoconversion, photobleaching |
| Analysis | Förster Resonance Energy Transfer (FRET) analysis |
| Filters | DAPI, FITC, CFP, Cy5, Rhod (5 filter sets) |
| Detectors | Power Hybrid Detectors |
| Imaging modes | Brightfield (BF) imaging |
| Image resolution | 8192 × 8192 pixels |
| 3D imaging | Z-stack acquisition for 3D imaging |
| Data depth | 16-bit data depth |
| Live-cell environment | Temperature- and humidity-controlled incubator setup + CO₂ control |
| Motorization | Fully motorized hardware |
Contact / Service Request
For service requests: you may email [email protected]. (Please specify the sample type, purpose, and preferred date range.)